Tested Applications
| Positive WB detected in | BGC-823 cells, HepG2 cells, HuH-7 cells, MKN-45 cells |
| Positive IHC detected in | mouse kidney tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21139-1-AP targets GALNT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15591 Product name: Recombinant human GALNT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 316-499 aa of BC038440 Sequence: EFKNFFYIISPGVTKVDYGDISSRVGLRHKLQCKPFSWYLENIYPDSQIPRHYFSLGEIRNVETNQCLDNMARKENEKVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLNGPVTMLKCHHLKGNQLWEYDPVKLTLQHVNSNQCLDKATEEDSQVPSIRDCNGSRSQQWLLRNVTLPEIF Predict reactive species |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) |
| Calculated Molecular Weight | 559 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC038440 |
| Gene Symbol | GALNT1 |
| Gene ID (NCBI) | 2589 |
| RRID | AB_3085639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10472 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
N-acetylgalactosaminyltransferase 1 (GALNT1) is a member of a large family of Golgi resident polypeptide GALNT that initiate mucin-type O-glycosylation by transfer of α-GalNAc from UDP-GalNAc to serine or threonine residues of proteins. GALNT1 is frequently up-regulated in HCC and gastric cancer, and higher GALNT1 expression levels are associated with poorer overall survival (PMID: 25730904, 36439876). The antibody against GALNT1 recognized a specific band at 64 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALNT1 antibody 21139-1-AP | Download protocol |
| WB protocol for GALNT1 antibody 21139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GALNT1 Polyclonal antibody (Cat# 21139-1-AP) has 1 citation to date. It was cited in Journal of Cancer Research and Clinical Oncology (2026), in a study identifying GALNT1 as a potential therapeutic target in breast cancer. The cited application was WB and IF in human samples, with RNAi validation. The research field is oncology, specifically breast cancer biology and glycosylation-related therapeutic targeting.
| Species | Application | Title |
|---|---|---|
J Cancer Res Clin Oncol GALNT1 emerges as a potential therapeutic target in breast cancer.
|
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5. The reviewer used it for both Western Blot and Immunohistochemistry on human samples and reported that it works well for both applications. Overall, the feedback indicates strong performance and reliability across these two techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (04-22-2026) | Works well for western blotting and IHC
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