GALNT1 Polyclonal antibody

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  WB analysis using 21139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21139-1-AP (GALNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GALNT1 Polyclonal Antibody for WB, ELISA

Cat No. 21139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GALNAC T1, GALNT1, pp GaNTase 1

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Tested Applications

• Positive WB detected in: BGC-823 cells, HepG2 cells, HuH-7 cells, MKN-45 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21139-1-AP targets GALNT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNT1 fusion protein Ag15591
• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1)
• Calculated Molecular Weight: 559 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC038440
• Gene Symbol: GALNT1
• Gene ID (NCBI): 2589
• RRID: AB_3085639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q10472
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-acetylgalactosaminyltransferase 1 (GALNT1) is a member of a large family of Golgi resident polypeptide GALNT that initiate mucin-type O-glycosylation by transfer of α-GalNAc from UDP-GalNAc to serine or threonine residues of proteins. GALNT1 is frequently up-regulated in HCC and gastric cancer, and higher GALNT1 expression levels are associated with poorer overall survival (PMID: 25730904, 36439876). The antibody against GALNT1 recognized a specific band at 64 kDa.