Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, MKN-45 cells |
| Positive IHC detected in | mouse pancreas tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17441-1-AP targets GALNT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11523 Product name: Recombinant human GALNT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-571 aa of BC041120 Sequence: FLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ Predict reactive species |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2) |
| Calculated Molecular Weight | 571 aa, 65 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC041120 |
| Gene Symbol | GALNT2 |
| Gene ID (NCBI) | 2590 |
| RRID | AB_3085523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10471 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GALNT2 antibody 17441-1-AP | Download protocol |
| IHC protocol for GALNT2 antibody 17441-1-AP | Download protocol |
| WB protocol for GALNT2 antibody 17441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GALNT2 Polyclonal antibody (Cat# 17441-1-AP) has 1 citation. It was used in IHC in a study published in Cell Biology and Toxicology (2023), investigating GALNT2 as a critical regulator of radioresistance in non-small cell lung cancer through an integrated multi-omics analysis. The research field encompasses cancer biology, glycosylation, and radiation resistance.
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol GALNT2, an O-glycosylating enzyme, is a critical regulator of radioresistance of non-small cell lung cancer: evidence from an integrated multi-omics analysis |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer tested the antibody on human cell lines and tissue, using it for both Western Blot and Immunohistochemistry. They reported that it works well for both applications, indicating strong performance and reliability in these common research techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (04-22-2026) | Works well for western blotting and IHC
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