Tested Applications
| Positive WB detected in | human liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17035-1-AP targets GALT in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10524 Product name: Recombinant human GALT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-379 aa of BC015045 Sequence: MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAVVDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWPYQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGSEAGANWDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYHLGQKDRETATIA Predict reactive species |
| Full Name | galactose-1-phosphate uridylyltransferase |
| Calculated Molecular Weight | 379 aa, 43 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC015045 |
| Gene Symbol | GALT |
| Gene ID (NCBI) | 2592 |
| RRID | AB_2109296 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07902 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALT antibody 17035-1-AP | Download protocol |
| WB protocol for GALT antibody 17035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GALT Polyclonal antibody (Cat# 17035-1-AP) has 3 citations published in Frontiers in Molecular Biosciences, Journal of Inherited Metabolic Disease, and PLoS One. The associated research fields include Golgi membrane protein dimerization and UDP-Gal biosynthesis, galactose metabolism and GALT enzyme activity in galactosemia, and chondrocyte gene expression related to osteoarthritis. All citations validated the antibody for WB and IF applications in human samples.
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Cytosolic UDP-Gal biosynthetic machinery is required for dimerization of SLC35A2 in the Golgi membrane and its interaction with B4GalT1 | ||
J Inherit Metab Dis Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK. | ||
PLoS One Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity. |
