GALT Polyclonal antibody

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  WB analysis of human liver using 17035-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17035-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17035-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17035-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 40x lens).

GALT Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17035-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

Gal 1 P uridylyltransferase, GALT

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

17035-1-AP targets GALT in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10524

  Product name: Recombinant human GALT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-379 aa of BC015045

  Sequence: MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAVVDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWPYQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGSEAGANWDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYHLGQKDRETATIA

• Full Name: galactose-1-phosphate uridylyltransferase
• Calculated Molecular Weight: 379 aa, 43 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC015045
• Gene Symbol: GALT
• Gene ID (NCBI): 2592
• RRID: AB_2109296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07902
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Inherit Metab Dis; Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK.; Authors - Sher Li Oh
human	WB	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human	WB,IF	Front Mol Biosci; Cytosolic UDP-Gal biosynthetic machinery is required for dimerization of SLC35A2 in the Golgi membrane and its interaction with B4GalT1; Authors - Wojciech Wiertelak