Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse liver tissue, pig liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66322-1-Ig targets GAMT in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16778 Product name: Recombinant human GAMT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-236 aa of BC016760 Sequence: MSAPSATPIFAPGENCSPAWGAAPAAYDAADTHLRILGKPVMERWETPYMHALAAAASSKGGRVLEVGFGMAIAASKVQEAPIDEHWIIECNDGVFQRLRDWAPRQTHKVIPLKGLWEDVAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKNHAFRLLKPGGVLTYCNLTSWGELMKSKYSDITIMFEETQVPALLEAGFRRENIRTEVMALVPPADCRYYAFPQMITPLVTKG Predict reactive species |
| Full Name | guanidinoacetate N-methyltransferase |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC016760 |
| Gene Symbol | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2881702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAMT antibody 66322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GAMT Monoclonal antibody (catalog number 66322-1-Ig) has 6 total citations. These appear in Food & Function, Scientific Reports (two studies), BMC Cardiovascular Disorders, bioRxiv, and Antioxidants. Associated research fields include reproductive toxicology (sodium valproate embryotoxicity), spermatogenesis and tight junctions, gastric cancer disulfidptosis, cardiovascular endothelial dysfunction, liver cancer mitochondrial/immune vulnerabilities, and boar sperm cryoinjury. Most citations used Western blot applications.
| Species | Application | Title |
|---|---|---|
Food Funct The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice | ||
Sci Rep Identification of disulfidptosis-related subtypes in gastric cancer and GAMT is a key gene during disulfidptosis
| ||
Sci Rep Intratesticular creatine maintains spermatogenesis by defining tight junctions
| ||
Antioxidants (Basel) LDHC Hyperacetylation Disrupts the Guanidinoacetic Acid-Creatine Axis Underlying Cryoinjury in Boar Sperm: Rescue by GAA Supplementation. |
