GANP Polyclonal antibody

• Featured Product
• KD/KO Validated

GANP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11054-1-AP

Synonyms

MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48

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Validation Data Gallery

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  WB analysis of mouse brain using 11054-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11054-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11054-1-AP

  IP result of anti-GANP (IP:11054-1-AP, 4ug; Detection:11054-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11054-1-AP (GANP antibody) at dilution of 1:10 (under 10x lens).

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  IF Staining of HepG2 using 11054-1-AP

  Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody 11054-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 11054-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GANP Polyclonal antibody (11054-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

11054-1-AP targets GANP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GANP fusion protein Ag1486
• Full Name: minichromosome maintenance complex component 3 associated protein
• Calculated Molecular Weight: 218 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC013285
• Gene Symbol: GANP
• Gene ID (NCBI): 8888
• RRID: AB_2266280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.

Publications

Species	Application	Title
human	WB	Nat Commun; GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.; Authors - Singh Shailendra Kumar SK ; KD Validated
human	WB	J Immunol; GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.; Authors - Maeda Kazuhiko K ; KD Validated
human	WB	Front Mol Neurosci; The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.; Authors - Chunqing Yang ; KD Validated
human,monkey	WB,IF	J Biol Chem; GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA.; Authors - Maeda Kazuhiko K