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GAPDHS Polyclonal antibody

GAPDHS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human and More (1)

Applications

WB, ELISA

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13937-1-AP targets GAPDHS in WB, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4988

Product name: Recombinant human GAPDHS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 58-408 aa of BC036373

Sequence: LPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Predict reactive species
Full Name glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Calculated Molecular Weight 45 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC036373
Gene Symbol GAPDHS
Gene ID (NCBI) 26330
RRIDAB_2107611
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).

Protocols

Product Specific Protocols
WB protocol for GAPDHS antibody 13937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GAPDHS Polyclonal antibody (Cat# 13937-1-AP) has 18 citations. These appear in journals including Theranostics, J Hazard Mater, J Med Chem, Int J Mol Med, Oxid Med Cell Longev, J Cell Mol Med, BMC Cancer, Front Cell Dev Biol, Sci Rep, Int Immunopharmacol, PLoS One, J Mol Histol, Oncol Lett, and J Assist Reprod Genet. Research fields span oncology (osteosarcoma, melanoma, colorectal/breast cancer, leukemia), liver fibrosis, reproductive biology and male fertility, cardiomyopathy, bone development, and inflammatory/metabolic signaling.

SpeciesApplicationTitle
humanWB

Theranostics

Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.

Authors - Xinchun Li
humanWB

Theranostics

Up-regulation of PCOLCE by TWIST1 promotes metastasis in Osteosarcoma.

Authors - Shang Wang
mouseWB

J Hazard Mater

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Authors - Zijun Peng
chickenWB

J Hazard Mater

Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens

Authors - Quan Mo
ratWB

Int J Mol Med

Fibroblast growth factor-21 alleviates hypoxia/reoxygenation injury in H9c2 cardiomyocytes by promoting autophagic flux.

Authors - Zhong Ren
mouseWB

Oxid Med Cell Longev

Withaferin A Exerts Preventive Effect on Liver Fibrosis through Oxidative Stress Inhibition in a Sirtuin 3-Dependent Manner.

Authors - Jingya Gu
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