GAPVD1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84343-3-RR (same clone as 84343-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84343-3-RR (GAPVD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84343-3-RR (same clone as 84343-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84343-3-RR (same clone as 84343-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84343-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84343-3-RR against Human GAPVD1 were performed. The affinity constant is 1.43 nM.

GAPVD1 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84343-3-PBS

Clone No.241609F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

241609F12, GAPEX5, GAPex-5, GTPase-activating protein and VPS9 domain-containing protein 1, KIAA1521

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Product Information

84343-3-PBS targets GAPVD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10492

  Product name: Recombinant human GAPVD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 280-629 aa of BC021119

  Sequence: MKSVMSGDQLREDRMALDNLLANLPPAKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRTNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMISENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESFLDWVAHLMIRMSGRSVPAPAHQASVLCPA

• Full Name: GTPase activating protein and VPS9 domains 1
• Calculated Molecular Weight: 1478 aa, 165 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC021119
• Gene Symbol: GAPVD1
• Gene ID (NCBI): 26130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14C86
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.