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GAS2L3 Polyclonal antibody, PBS Only (25409-1-PBS)

Filter: AllWBIPIHC

WB analysis using 25409-1-AP (same clone as 25409-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 25409-1-AP (GAS2L3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 25409-1-AP (same clone as 25409-1-PBS)
IP result of anti-GAS2L3 (IP:25409-1-AP, 4ug; Detection:25409-1-AP 1:1000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25409-1-AP (GAS2L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 25409-1-AP (same clone as 25409-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25409-1-AP (GAS2L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25409-1-PBS in a different storage buffer formulation.

GAS2L3 Polyclonal antibody, PBS Only

Name: GAS2L3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 25409-1-PBS

GAS2L3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 25409-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GAS2 like protein 3, growth arrest 2 like 3

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

25409-1-PBS targets GAS2L3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22097 Product name: Recombinant human GAS2L3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 347-694 aa of BC043366 Sequence: GRPPGALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPALPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLAAHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNLNQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPKSAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK Predict reactive species
Full Name	growth arrest-specific 2 like 3
Calculated Molecular Weight	694 aa, 75 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC043366
Gene Symbol	GAS2L3
Gene ID (NCBI)	283431
RRID	AB_3669473
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86XJ1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GAS2L3 also known as G2L3, belongs to the GAS2 family of four related proteins. It contains an N-terminal calponin homology (CH) domain and a C-terminal GAS2-related (GAR) domain, which are putative actin and microtubule binding domains. GAS2L3 is specifically expressed in mitosis and it localizes to the midbody and the constriction zone during cytokinesis (PMID: 28937871).