GAS6 Polyclonal antibody

GAS6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13795-1-AP

Synonyms

AXLLG, AXSF, GAS 6, GAS6, growth arrest 6



Tested Applications

Positive WB detected inhuman urine sample, DU 145 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13795-1-AP targets GAS6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAS6 fusion protein Ag4617
Full Name growth arrest-specific 6
Calculated Molecular Weight 678 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC038984
Gene Symbol GAS6
Gene ID (NCBI) 2621
RRIDAB_2756522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth arrest-specific protein 6 (GAS6) is also named as AXLLG. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses (PMID:12364394, PMID:18840707). It can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PMID:21501828).

Protocols

Product Specific Protocols
WB protocol for GAS6 antibody 13795-1-APDownload protocol
IHC protocol for GAS6 antibody 13795-1-APDownload protocol
IF protocol for GAS6 antibody 13795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.

Authors - Yingying Shen
humanWB

Front Cell Infect Microbiol

Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.

Authors - Fengxue Geng
humanWB

FASEB J

Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases.

Authors - Hua Qian
humanWB,IHC

Cancer Gene Ther

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Authors - Minhai Dong
mouseWB

Transplantation

Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.

Authors - Zhen Wang
humanWB

Mol Immunol

The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury

Authors - Yujing Cheng