GATA1 Monoclonal antibody

• Featured Product
• KD/KO Validated

GATA1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

5E2A8

Cat no : 60011-1-Ig

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

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Validation Data Gallery

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  WB analysis using 60011-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60011-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 60011-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug GATA1 antibody (60011-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HL-60 cells, Raji cells, K-562 cells, Jurkat cells
• Positive IHC detected in: human breast hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

60011-1-Ig targets GATA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA1 fusion protein Ag1350
• Full Name: GATA binding protein 1 (globin transcription factor 1)
• Calculated molecular weight: 43 kDa
• Observed molecular weight: 43 kDa
• GenBank accession number: BC009797
• Gene symbol: GATA1
• Gene ID (NCBI): 2623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Publications

Species	Application	Title
human	IP	Blood; PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.; Authors - Wu Jie J
human	IF	Development; The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.; Authors - T Michael Creed ; KO Validated
human	WB	Biochem J; ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.; Authors - Chen Jing J
human	WB	J Cell Mol Med; 1,25(OH)2 D3 blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus.; Authors - Xuejun Ge
human	WB	Cell Death Discov; JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.; Authors - Yanlin Ren
human	WB	Cell Death Dis; GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.; Authors - Wenqian Yu