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  • KD/KO Validated

GATA1 Monoclonal antibody

GATA1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E2A8

Cat no : 60011-1-Ig

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT



Tested Applications

Positive WB detected inHL-60 cells, Raji cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60011-1-Ig targets GATA1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GATA1 fusion protein Ag1350
Full Name GATA binding protein 1 (globin transcription factor 1)
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC009797
Gene Symbol GATA1
Gene ID (NCBI) 2623
RRIDAB_2294437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Protocols

Product Specific Protocols
WB protocol for GATA1 antibody 60011-1-IgDownload protocol
IHC protocol for GATA1 antibody 60011-1-IgDownload protocol
FC protocol for GATA1 antibody 60011-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Blood

PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.

Authors - Wu Jie J
humanIF

Development

The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.

Authors - T Michael Creed
  • KO Validated
humanWB

Biochem J

ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.

Authors - Chen Jing J
humanWB

J Cell Mol Med

1,25(OH)2 D3 blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus.

Authors - Xuejun Ge
humanWB

Cell Death Discov

JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.

Authors - Yanlin Ren
humanWB

Cell Death Dis

GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.

Authors - Wenqian Yu