Validation Data Gallery
|Positive WB detected in||HL-60 cells, Raji cells, K-562 cells, Jurkat cells|
|Positive IHC detected in||human breast hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
60011-1-Ig targets GATA1 in WB, IP, IHC, IF, FC applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GATA1 fusion protein Ag1350|
|Full Name||GATA binding protein 1 (globin transcription factor 1)|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC009797|
|Gene ID (NCBI)||2623|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].
Cell Death Dis
GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.
Cell Death Discov
JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.
ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.
The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.
PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.