GATA6 Recombinant monoclonal antibody

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  WB analysis using 84987-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84987-5-RR (GATA6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84987-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GATA6 antibody (84987-5-RR, Clone: 242325G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84987-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA6 antibody (84987-5-RR, Clone: 242325G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GATA6 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84987-5-RR

Clone No.242325G7

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

GATA-binding factor 6, Transcription factor GATA 6, Transcription factor GATA-6

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, SGC-7901 cells
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

84987-5-RR targets GATA6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GATA binding protein 6
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: NM_005257
• Gene Symbol: GATA6
• Gene ID (NCBI): 2627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Commun Signal; miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby.; Authors - Nikoletta Kalenderoglou