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GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 27299-1-AP

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2



Tested Applications

Positive WB detected inA375 cells, HeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27299-1-AP is GBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP2 fusion protein Ag25433
Full Name guanylate binding protein 2, interferon-inducible
Calculated molecular weight 591 aa, 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC022272
Gene symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GBP2 antibody 27299-1-APDownload protocol
IHC protocol for GBP2 antibody 27299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response.

Authors - Sannula Kesavardhana
humanWB,IF

J Immunol

TLR and IKK Complex-Mediated Innate Immune Signaling Inhibits Stress Granule Assembly.

Authors - Parimal Samir
mouseWB

J Immunol

IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.

Authors - Rajendra Karki