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  • KD/KO Validated

GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13220-1-AP

Synonyms

GBP 5, GBP TA antigen, GBP5, GTP binding protein 5, guanylate binding protein 5



Tested Applications

Positive WB detected inU-937 cells, Jurkat cells
Positive IP detected inU-937 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP5 fusion protein Ag4103
Full Name guanylate binding protein 5
Calculated molecular weight 586 aa, 67 kDa
Observed molecular weight 65 kDa, 55 kDa
GenBank accession numberBC031639
Gene symbol GBP5
Gene ID (NCBI) 115362
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Protocols

Product Specific Protocols
WB protocol for GBP5 antibody 13220-1-APDownload protocol
IP protocol for GBP5 antibody 13220-1-APDownload protocol
FC protocol for GBP5 antibody 13220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

GBP5 drives malignancy of glioblastoma via the Src/ERK1/2/MMP3 pathway.

Authors - Xiaoting Yu
  • KD Validated
humanWB

Cytokine

Cross-talk between SUMOylation and ISGylation in response to interferon.

Authors - Faten El-Asmi
humanIHC

Front Genet

Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System.

Authors - Xue Wang
mouseWB

Cell Microbiol

Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.

Authors - Fernanda L Tana
humanIHC

Histopathology

Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma.

Authors - Pallavi A Patil
mouseWB,IHC

Mol Pharm

Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma.

Authors - Tong-Fei Li