Jurkat cells were subjected to SDS PAGE followed by western blot with 13220-1-AP(GBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 13220-1-AP( GBP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP( GBP5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-GBP5 (IP:13220-1-AP, 4ug; Detection:13220-1-AP 1:1000) with U-937 cells lysate 4000ug.

Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody 13220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 MCF-7 cells were stained with 0.2ug GBP5 antibody (13220-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.