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- KD/KO Validated
GBP5 Polyclonal antibody
GBP5 Polyclonal Antibody for FC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 13220-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-937 cells, Jurkat cells |
Positive IP detected in | U-937 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 26 publications below |
IHC | See 10 publications below |
IF | See 7 publications below |
Product Information
13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GBP5 fusion protein Ag4103 |
Full Name | guanylate binding protein 5 |
Calculated molecular weight | 586 aa, 67 kDa |
Observed molecular weight | 65 kDa, 55 kDa |
GenBank accession number | BC031639 |
Gene symbol | GBP5 |
Gene ID (NCBI) | 115362 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)
Protocols
Product Specific Protocols | |
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WB protocol for GBP5 antibody 13220-1-AP | Download protocol |
IP protocol for GBP5 antibody 13220-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis GBP5 drives malignancy of glioblastoma via the Src/ERK1/2/MMP3 pathway.
| ||
Inflammation Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ. | ||
Front Genet Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System. | ||
Cell Microbiol Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice. | ||
Histopathology Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma. |