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GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13220-1-AP

Synonyms

GBP, GBP 5, GBP TA antigen, GBP5, GTP binding protein 5, guanylate binding protein 5



Tested Applications

Positive WB detected inU-937 cells, Jurkat cells
Positive IP detected inU-937 cells
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP5 fusion protein Ag4103
Full Name guanylate binding protein 5
Calculated molecular weight 586 aa, 67 kDa
Observed molecular weight 65 kDa, 55 kDa
GenBank accession numberBC031639
Gene symbol GBP5
Gene ID (NCBI) 115362
RRIDAB_2109348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Protocols

Product Specific Protocols
WB protocol for GBP5 antibody 13220-1-APDownload protocol
IHC protocol for GBP5 antibody 13220-1-APDownload protocol
IP protocol for GBP5 antibody 13220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Authors - Peng Li
mouseWB

Nature

Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

Authors - Etienne Meunier
mouseWB

Cell

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Authors - Rajendra Karki
humanWB

Cell

IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

Authors - Si Ming Man
mouseWB

Nat Immunol

The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

Authors - Si Ming Man
mouseWB

Nat Immunol

Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.

Authors - Etienne Meunier
  • KO Validated