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GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13220-1-AP

Synonyms

GBP 5, GBP TA antigen, GBP5, GTP binding protein 5, guanylate binding protein 5



Tested Applications

Positive WB detected inU-937 cells, Jurkat cells
Positive IP detected inU-937 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP5 fusion protein Ag4103
Full Name guanylate binding protein 5
Calculated molecular weight 586 aa, 67 kDa
Observed molecular weight 65 kDa, 55 kDa
GenBank accession numberBC031639
Gene symbol GBP5
Gene ID (NCBI) 115362
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Protocols

Product Specific Protocols
WB protocol for GBP5 antibody 13220-1-APDownload protocol
IP protocol for GBP5 antibody 13220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Authors - Peng Li
mouseWB,IF

Nature

Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

Authors - Etienne Meunier
mouseWB

Cell

IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Authors - Rajendra Karki
humanWB,IF

Cell

IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

Authors - Si Ming Man
mouseWB, IF

Nat Immunol

The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

Authors - Si Ming Man
mouseWB,IF

Nat Immunol

Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.

Authors - Etienne Meunier
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