GBP5 Polyclonal antibody

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  WB analysis using 13220-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP (GBP5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13220-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13220-1-AP (GBP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 13220-1-AP

  IP result of anti-GBP5 (IP:13220-1-AP, 4ug; Detection:13220-1-AP 1:1000) with U-937 cells lysate 4000 ug.

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  IHC staining of rat spleen using 13220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13220-1-AP (GBP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 13220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13220-1-AP (GBP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 13220-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug GBP5 Polyclonal antibody (13220-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GBP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13220-1-AP

Publications(44)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5

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Tested Applications

• Positive WB detected in: U-937 cells, Jurkat cells
• Positive IP detected in: U-937 cells
• Positive IHC detected in: rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 37 publications below
• IHC: See 17 publications below
• IF: See 1 publications below

Product Information

13220-1-AP targets GBP5 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP5 fusion protein Ag4103
• Full Name: guanylate binding protein 5
• Calculated Molecular Weight: 586 aa, 67 kDa
• Observed Molecular Weight: 65 kDa, 55 kDa
• GenBank Accession Number: BC031639
• Gene Symbol: GBP5
• Gene ID (NCBI): 115362
• RRID: AB_2109348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PP8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Publications

Species	Application	Title
human	WB	Nature; Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.; Authors - Peng Li
	WB	Nature; Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.; Authors - Etienne Meunier
	WB	Cell; IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.; Authors - Rajendra Karki
human	WB	Cell; IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.; Authors - Si Ming Man
	WB,IF	Nat Immunol; The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.; Authors - Si Ming Man
	WB	Nat Immunol; Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.; Authors - Etienne Meunier ; KO Validated