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GBP5 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 13220-1-AP

Featured Product KD/KO validated

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Con: 64 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

GBP5 Antibody WB U-937 cells 13220-1-AP

U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP( GBP5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

GBP5 Antibody IHC human prostate cancer tissue 13220-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 10x lens)

GBP5 Antibody IHC human prostate cancer tissue 13220-1-AP

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 40x lens)

GBP5 Antibody IF MCF-7 cells 13220-1-AP

Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody 13220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GBP5 Antibody IP U-937 cells 13220-1-AP

IP result of anti-GBP5(IP:13220-1-AP, 4ug; Detection:13220-1-AP 1:1000) with U-937 cells lysate 4000 ug

GBP5 Antibody FC MCF-7 cells 13220-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug GBP5 antibody (13220-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse
Positive WB detected in:
U-937 cells, Jurkat cells
Positive IP detected in:
U-937 cells
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GBP5 fusion protein Ag4103
Full name:
guanylate binding protein 5
Calculated molecular weight:
586aa,67 kDa
Observed molecular weight:
65 kDa, 55 kDa
GenBank accession number:
BC031639
Gene ID (NCBI):
115362
Gene symbol
GBP5
Synonyms
GBP 5, GBP TA antigen, GBP5, GTP binding protein 5, guanylate binding protein 5

Background

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1βand IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa).


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