|Positive WB detected in
|U-937 cells, Jurkat cells
|Positive IP detected in
|Positive IHC detected in
|rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:250-1:1000
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13220-1-AP targets GBP5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GBP5 fusion protein Ag4103
|guanylate binding protein 5
|Calculated molecular weight
|586 aa, 67 kDa
|Observed molecular weight
|65 kDa, 55 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)
Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.
Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.