GBP5 Polyclonal antibody

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• KD/KO Validated

GBP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13220-1-AP Copied!

Publications (58)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA
For IF or FC, select conjugated version.

Synonyms

EC:3.6.5.-, GBP-5, GBP-TA antigen, GTP-binding protein 5, Guanine nucleotide-binding protein 5

• 

  WB analysis using 13220-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP (GBP5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 13220-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13220-1-AP (GBP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of U-937 using 13220-1-AP

  IP result of anti-GBP5 (IP:13220-1-AP, 4ug; Detection:13220-1-AP 1:1000) with U-937 cells lysate 4000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 13220-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13220-1-AP (GBP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat spleen using 13220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 13220-1-AP (GBP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 13220-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug GBP5 Polyclonal antibody (13220-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: U-937 cells, Jurkat cells
• Positive IP detected in: U-937 cells
• Positive IHC detected in: human ovary tumor tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13220-1-AP targets GBP5 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4103

  Product name: Recombinant human GBP5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 382-586 aa of BC031639

  Sequence: LETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHAQRTVNNDDPCVLL

• Full Name: guanylate binding protein 5
• Calculated Molecular Weight: 586 aa, 67 kDa
• Observed Molecular Weight: 65 kDa, 55 kDa
• GenBank Accession Number: BC031639
• Gene Symbol: GBP5
• Gene ID (NCBI): 115362
• RRID: AB_2109348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PP8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

Publications

What published studies show

The anti-GBP5 antibody (Cat# 13220-1-AP) has 58 citations spanning journals including Nature, Cell, Nature Immunology, Nature Microbiology, EMBO Journal, and PLoS Pathogens. Research fields cover innate immunity and inflammasome activation, intracellular infections (bacterial, viral, fungal, parasitic), cancer immunology (glioblastoma, breast cancer, lung cancer, oral squamous cell carcinoma), macrophage biology, ferroptosis, cGAS-STING signaling, autoimmune/inflammatory diseases, and nanomedicine-based drug delivery.

Automatically generated from the 58 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nature; Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.; Authors - Etienne Meunier
human	WB	Nature; Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.; Authors - Peng Li
	WB	Cell; IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.; Authors - Rajendra Karki
human	WB	Cell; IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.; Authors - Si Ming Man
	WB,IF	Nat Immunol; The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.; Authors - Si Ming Man
	WB	Nat Immunol; Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.; Authors - Etienne Meunier ; KO Validated