GCC2 Polyclonal antibody, PBS Only

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• GCC2 Polyclonal antibody, PBS Only (32217-1-PBS)

• 

  WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32217-1-AP (GCC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 32217-1-AP (same clone as 32217-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32217-1-AP (GCC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 32217-1-AP (same clone as 32217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 32217-1-AP (GCC2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 32217-1-AP (same clone as 32217-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 32217-1-AP (GCC2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32217-1-PBS in a different storage buffer formulation.

GCC2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 32217-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC185, GRIP And Coiled-Coil Domain Containing 2

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Product Information

32217-1-PBS targets GCC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17535

  Product name: Recombinant human GCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-343 aa of BC146789

  Sequence: MKQEVEDSVTKMGDAHKELEQSHINYVKEIENLKNELMAVRSKYSEDKANLQKQLEEAMNTQLELSEQLKFQNNSEDNVKKLQEEIEKIRPGFEEQILYLQKQLDATTDEKKETVTQLQNIIEANSQHYQKNINSLQEELLQLKAIHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKATSNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKELESQHSILKDEVTYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGCVIEKLKSELAGLNKQFCYTVEQHNREVQSLKEQHQKEISELNETF

• Full Name: GRIP and coiled-coil domain containing 2
• Calculated Molecular Weight: 1583 aa, 185 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC146789
• Gene Symbol: GCC2
• Gene ID (NCBI): 9648
• RRID: AB_3742546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8IWJ2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The GCC2 protein, fully named GRIP and Coiled-Coil Domain-Containing Protein 2, is a key adaptor protein in the intracellular Golgi-endosomal membrane transport system. It specifically anchors to the Golgi membrane via its GRIP domain and is primarily responsible for mediating retrograde vesicular transport from endosomes to the Golgi apparatus, participating in the recycling and sorting of various cargo proteins, such as the mannose-6-phosphate receptor. Recent studies have revealed that GCC2 regulates the subcellular localization of the GATOR complex, thereby acting as an important upstream regulator of the mTORC1 signaling pathway, which influences cell growth, metabolism, and autophagy. Dysfunction of GCC2 is closely associated with neurodevelopmental disorders and tumorigenesis, underscoring its significance in cell biology and disease research.