GCH1 Polyclonal antibody

GCH1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28501-1-AP

Synonyms

DYT14, DYT5, GCH, GCH1, GTP CH 1, GTP CH I, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTPCH1



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inrat liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28501-1-AP targets GCH1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCH1 fusion protein Ag28896
Full Name GTP cyclohydrolase 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC025415
Gene Symbol GCH1
Gene ID (NCBI) 2643
RRIDAB_2881157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GCH1 antibody 28501-1-APDownload protocol
IHC protocol for GCH1 antibody 28501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Res

3,6-Epidioxy-1,10-bisaboladiene induces ferroptosis-like cell death through lipid peroxidation

Authors - Narandulam Usukhbayar
mouse,humanWB

Nat Cell Biol

Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition

Authors - Weiwei Cui
humanWB

Eur Rev Med Pharmacol Sci

Identification of ferroptosis-related genes for the prediction of prognosis and chemotherapy benefit of gastric cancer

Authors - Jiujian Zheng
humanWB,IF

Virol Sin

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1

Authors - Tanxiu Chen
  • KD Validated
humanWB

Adv Sci (Weinh)

Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth

Authors - Dong Liu
  • KO Validated