GCLC Polyclonal antibody, PBS Only

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  WB analysis using 12601-1-AP (same clone as 12601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 12601-1-AP (same clone as 12601-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12601-1-AP (same clone as 12601-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GCLC Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12601-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

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Product Information

12601-1-PBS targets GCLC in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3252

  Product name: Recombinant human GCLC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-252 aa of BC022487

  Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV

• Full Name: glutamate-cysteine ligase, catalytic subunit
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC022487
• Gene Symbol: GCLC
• Gene ID (NCBI): 2729
• RRID: AB_2278734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48506
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).