GCLM Monoclonal antibody

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GCLM Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66808-1-Ig Copied!

Clone No. 1A5A4

Publications (7)

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Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

1A5A4, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit

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  WB analysis using 66808-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 66808-1-Ig

  WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66808-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66808-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66808-1-Ig targets GCLM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5497

  Product name: Recombinant human GCLM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-274 aa of BC041809

  Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

• Full Name: glutamate-cysteine ligase, modifier subunit
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC041809
• Gene Symbol: GCLM
• Gene ID (NCBI): 2730
• RRID: AB_2882151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48507
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Nrf2 antibody (Cat# 66808-1-Ig) has 7 total citations across journals including J Transl Med, Free Radic Biol Med, Oxid Med Cell Longev, Eur J Med Chem, Mar Drugs, J Enzyme Inhib Med Chem, and Front Cell Dev Biol. Research fields span ferroptosis, oxidative stress, liver injury, Alzheimer's disease, NAFLD, cuproptosis, and radioresistance in esophageal carcinoma and hepatocellular carcinoma.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Transl Med; Long noncoding RNA PCAT7 confers radioresistance of esophageal squamous cell carcinoma by promoting GCLM-mediated anti-ferroptosis.; Authors - Yu Hao
human,mouse	WB	Free Radic Biol Med; An intrabody targeting PHD2 protects mice against acetaminophen-induced liver injury by attenuating ferroptosis.; Authors - Ze Ren
human	WB	Oxid Med Cell Longev; Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.; Authors - Ning Ma
rat	WB	Eur J Med Chem; Design, synthesis and evaluation of fused hybrids with acetylcholinesterase inhibiting and Nrf2 activating functions for Alzheimer's disease; Authors - Yuanyuan Wang
human	WB	Mar Drugs; Fucoxanthin Attenuates Free Fatty Acid-Induced Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism/Oxidative Stress/Inflammation via the AMPK/Nrf2/TLR4 Signaling Pathway; Authors - Jiena Ye
human	WB	Front Cell Dev Biol; Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma; Authors - Feng Cao