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GCLM Monoclonal antibody
GCLM Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A5A4
Cat no : 66808-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
Product Information
66808-1-Ig targets GCLM in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | GCLM fusion protein Ag5497 |
Full Name | glutamate-cysteine ligase, modifier subunit |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC041809 |
Gene Symbol | GCLM |
Gene ID (NCBI) | 2730 |
RRID | AB_2882151 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for GCLM antibody 66808-1-Ig | Download protocol |
IHC protocol for GCLM antibody 66808-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. | ||
Eur J Med Chem Design, synthesis and evaluation of fused hybrids with acetylcholinesterase inhibiting and Nrf2 activating functions for Alzheimer's disease | ||
Front Cell Dev Biol Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma | ||
Mar Drugs Fucoxanthin Attenuates Free Fatty Acid-Induced Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism/Oxidative Stress/Inflammation via the AMPK/Nrf2/TLR4 Signaling Pathway |