GCLM Monoclonal antibody, PBS Only

• 

  WB analysis using 66808-1-Ig (same clone as 66808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66808-1-Ig (same clone as 66808-1-PBS)

  WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GCLM Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66808-1-PBS

Clone No.1A5A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1A5A4, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit

---

Product Information

66808-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5497

  Product name: Recombinant human GCLM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-274 aa of BC041809

  Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

• Full Name: glutamate-cysteine ligase, modifier subunit
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC041809
• Gene Symbol: GCLM
• Gene ID (NCBI): 2730
• RRID: AB_2882151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48507
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.