GCM1 Polyclonal antibody

GCM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21724-1-AP

Synonyms

GCM motif protein 1, GCM1, GCMA, Glial cells missing homolog 1, hGCMa

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Validation Data Gallery

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  WB analysis of HepG2 using 21724-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21724-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21724-1-AP (GCM1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 21724-1-AP is GCM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCM1 fusion protein Ag16383
• Full Name: glial cells missing homolog 1 (Drosophila)
• Calculated Molecular Weight: 436 aa, 49 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC096288
• Gene Symbol: GCM1
• Gene ID (NCBI): 8521
• RRID: AB_2878913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Reprod Biol Endocrinol; Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.; Authors - Wei Peng
human	WB	BMC Med; Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth; Authors - Jiayi Wu