Product Information
30993-1-PBS targets GCN1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34311 Product name: Recombinant human GCN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 624-745 aa of BC153881 Sequence: EALVTDAGEVTEAGKAYVPPRVLQEALCVISGVPGLKGDVTDTEQLAQEMLIISHHPSLVAVQSGLWPALLARMKIDPEAFITRHLDQIIPRMTTQSPLNQSSMNAMGSLSVLSPDRVLPQLIST Predict reactive species |
| Full Name | GCN1 general control of amino-acid synthesis 1-like 1 (yeast) |
| Calculated Molecular Weight | 293 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC153881 |
| Gene Symbol | GCN1L1 |
| Gene ID (NCBI) | 10985 |
| RRID | AB_3742385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q92616 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
GCN1 (General Control Nonderepressible 1), also designated as GCN1L1, is a member of the GCN1 protein family. Its core function is to serve as a central regulator of the integrated stress response (ISR), acting as a key molecular adaptor that facilitates the activation of GCN2 kinase. Under stress conditions such as amino acid starvation, GCN1 binds uncharged tRNA through its C-terminal domain while concurrently binding and activating GCN2 via its N-terminal domain. This leads to the phosphorylation of eIF2α, thereby initiating the ISR to modulate translation and gene expression. Furthermore, GCN1 participates in ribosome-associated quality control (RQC). In coordination with the RQT complex, it mediates the ubiquitination and subsequent degradation of aberrant nascent polypeptide chains on stalled ribosomes, thus preserving protein homeostasis.
