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GCNA Polyclonal antibody, PBS Only (30693-1-PBS)

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WB analysis using 30693-1-AP (same clone as 30693-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 30693-1-AP (ACRC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.
WB analysis using 30693-1-AP (same clone as 30693-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 30693-1-AP (GCNA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 30693-1-AP (GCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 30693-1-AP (GCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.

GCNA Polyclonal antibody, PBS Only

Name: GCNA Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 30693-1-PBS

GCNA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 30693-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ACRC, Acidic repeat-containing protein, Germ cell nuclear acidic peptidase, Germ cell nuclear acidic protein, Germ cell nuclear antigen

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

30693-1-PBS targets GCNA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag33720 Product name: Recombinant human ACRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC136259 Sequence: MDGCKKELPRLQEPEEDEDCYILNVQSSSDDTSGSSVARRAPKRQASCILNVQSRSGDTSGSSVARRAPKRQASSVVVIDSDSDEECHTHEEKKAKLLEINSDDESPECCHVKPAIQEPPIVISDDDNDDDNGNDLE Predict reactive species
Full Name	acidic repeat containing
Calculated Molecular Weight	691 aa, 76 kDa
Observed Molecular Weight	76 kDa
GenBank Accession Number	BC136259
Gene Symbol	GCNA
Gene ID (NCBI)	93953
RRID	AB_3086389
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96QF7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Germ cell nuclear acidic protein (GCNA) has been extensively used as a germ cell marker for almost 30 years. Recently, mutations in GCNA have been linked to azoospermia in humans, defining GCNA as a clinical determinant of human infertility.