GEMIN5 Polyclonal antibody

GEMIN5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (2)

Cat no : 24897-1-AP

Synonyms

Gem associated protein 5, GEMIN5

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Validation Data Gallery

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  WB analysis using 24897-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24897-1-AP

  IP result of anti-GEMIN5 (IP:24897-1-AP, 4ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of mouse cerebellum using 24897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 24897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24897-1-AP

  Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

24897-1-AP targets GEMIN5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEMIN5 fusion protein Ag19268
• Full Name: gem (nuclear organelle) associated protein 5
• Calculated Molecular Weight: 1508 aa, 169 kDa
• Observed Molecular Weight: 168 kDa
• GenBank Accession Number: BC113614
• Gene Symbol: GEMIN5
• Gene ID (NCBI): 25929
• RRID: AB_2879784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

Publications

Species	Application	Title
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
human	WB	Front Cell Dev Biol; Autosomal Recessive Cerebellar Atrophy and Spastic Ataxia in Patients With Pathogenic Biallelic Variants in GEMIN5.; Authors - Deepa S Rajan
human	WB,IF	FEBS Lett; Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.; Authors - Dongxu Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Céline (Verified Customer) (11-28-2024)

Good for Western blot and Immunofluorescence applications

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Hela cells

FH

Parisa (Verified Customer) (06-13-2022)

The first lane shows the Gemin5 in HEK293 cell line and the second one is in the KD cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

GEMIN5 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:24897-1-AP)