Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human ovary cancer tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12307-2-AP targets GEMIN6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2954 Product name: Recombinant human GEMIN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC018195 Sequence: MSEWMKKGPLEWQDYIYKEVRVTASEKNEYKGWVLTTDPVSANIVLVNFLEDGSMSVTGIMGHAVQTVETMNEGDHRVREKLMHLFTSGDCKAYSPEDLEERKNSLKKWLEKNHIPITEQGDAPRTLCVAGVLTIDPPYDPENCSSSNEIILSRVQDLIEGHLTASQ Predict reactive species |
| Full Name | gem (nuclear organelle) associated protein 6 |
| Calculated Molecular Weight | 167 aa, 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC018195 |
| Gene Symbol | GEMIN6 |
| Gene ID (NCBI) | 79833 |
| RRID | AB_2111835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GEMIN6, also named as SIP2, is a subunit of SMN complex. It plays an important role in the splicing of cellular pre-mRNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GEMIN6 antibody 12307-2-AP | Download protocol |
| IHC protocol for GEMIN6 antibody 12307-2-AP | Download protocol |
| WB protocol for GEMIN6 antibody 12307-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GEMIN6 Polyclonal antibody (Cat# 12307-2-AP) has 3 total citations. These appear in Nucleic Acids Research (2 papers) and Oncogene (1 paper). The cited research spans RNA biology and cancer: two studies in Nucleic Acids Research investigated the SMN complex's role in selenoprotein mRNP assembly and U4-specific protein processing, while the Oncogene paper explored GEMIN6's involvement in TFE3-rearranged renal cell carcinoma progression. All citations used the antibody for Western blot, with one also employing IHC.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins. | ||
Nucleic Acids Res SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation. | ||
Oncogene LGI3 promotes the progression of TFE3-rearranged renal cell carcinoma through GEMIN6/AURKB axis |
Reviews
What customers say
The antibody received a 5-star rating for immunofluorescence on HeLa cells at a 1:200 dilution. The reviewer described it as very bright and highly specific, with no background observed. Fixation was performed with paraformaldehyde, and cells were permeabilized with 0.1% Triton X-100. Overall, the feedback highlights strong signal intensity and clean specificity in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Céline (Verified Customer) (01-20-2026) | It is a very bright and very specific antibody. Ther was no background. Fixation was done with paraformaldehyde and cells were permeabilized with 0,1% tritonX100.
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