GEMIN8 Polyclonal antibody

GEMIN8 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15251-1-AP

Synonyms

FAM51A1, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1

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Validation Data Gallery

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  WB analysis of HepG2 using 15251-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15251-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15251-1-AP

  IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug.

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  IHC staining of human liver cancer using 15251-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 15251-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15251-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15251-1-AP targets GEMIN8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEMIN8 fusion protein Ag6774
• Full Name: gem (nuclear organelle) associated protein 8
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC003607
• Gene Symbol: GEMIN8
• Gene ID (NCBI): 54960
• RRID: AB_2111845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.; Authors - Anne-Sophie Gribling-Burrer