GFPT2 Polyclonal antibody

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  WB analysis of HT1080 cells using 15189-1-AP

  WB redult of GFPT2 antibody (15189-1-AP) with HT1080 cells.

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  IP experiment of HT-1080 using 15189-1-AP

  IP result of anti-GFPT2 (IP:15189-1-AP, 4ug; Detection:15189-1-AP 1:300) with HT-1080 cells lysate 2400ug.

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  IHC staining of human ovary cancer using 15189-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15189-1-AP (GFPT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 15189-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15189-1-AP (GFPT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GFPT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15189-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

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Tested Applications

• Positive WB detected in: HT1080 cells
• Positive IP detected in: HT-1080 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15189-1-AP targets GFPT2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFPT2 fusion protein Ag7275
• Full Name: glutamine-fructose-6-phosphate transaminase 2
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 75-77 kDa
• GenBank Accession Number: BC000012
• Gene Symbol: GFPT2
• Gene ID (NCBI): 9945
• RRID: AB_2878116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94808
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

Publications

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Sci China Life Sci; Deamidation enables pathogenic SMAD6 variants to activate the BMP signaling pathway; Authors - Ling Li
mouse	WB	Prog Neuropsychopharmacol Biol Psychiatry; Dihydroartemisinin promotes tau O-GlcNAcylation and improves cognitive function in hTau transgenic mice; Authors - Lei Xia