GGCT Polyclonal antibody, PBS Only

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• GGCT Polyclonal antibody, PBS Only (16257-1-PBS)

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  WB analysis using 16257-1-AP (same clone as 16257-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 16257-1-AP (same clone as 16257-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 16257-1-AP (same clone as 16257-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 16257-1-AP (same clone as 16257-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16257-1-AP (same clone as 16257-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 16257-1-AP (same clone as 16257-1-PBS)

  IP result of anti-GGCT (IP:16257-1-AP, 4ug; Detection:16257-1-AP 1:300) with mouse kidney tissue lysate 3000 ug. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 16257-1-AP (same clone as 16257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 16257-1-AP (same clone as 16257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16257-1-AP (same clone as 16257-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16257-1-AP (GGCT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

GGCT Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16257-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

16257-1-PBS targets GGCT in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9284

  Product name: Recombinant human GGCT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-188 aa of BC019243

  Sequence: MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL

• Full Name: gamma-glutamyl cyclotransferase
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC019243
• Gene Symbol: GGCT
• Gene ID (NCBI): 79017
• RRID: AB_10852962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75223
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.