Anti-GGGGS Linker Rabbit Recombinant Antibody, PBS Only

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  WB analysis using 98262-1-RR (same clone as 98262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IHC staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected CHO-K1 using 98262-1-RR (same clone as 98262-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected CHO-K1 using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells (left) or anti-CD19 (FMC63 Scfv-G4S*2) CAR-293T cell line (right) were surface stained with 0.25 ug Anti-GGGGS Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells (left) or anti-CD19 (FMC63 Scfv-G4S*3) CAR-293T cell line (right) were surface stained with 0.25 ug Anti-GGGGS Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells (left) or anti-CD19 (FMC63 Scfv-G4S*4) CAR-293T cell line (right) were surface stained with 0.25 ug Anti-GGGGS Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells (left) or anti-CD19 (FMC63 Scfv-G4S*5) CAR-293T cell line (right) were surface stained with 0.25 ug Anti-GGGGS Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98262-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98262-1-RR against scFv (single chain fragment variable) format with variable regions of heavy (VH) and light (VL) chains joined together by G4S linker were performed. The affinity constant is 3.87 nM.

GGGGS Linker Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC

Cat No. 98262-1-PBS

Clone No.242306C8

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IHC, IF/ICC, FC

Synonyms

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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Product Information

98262-1-PBS targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GGGGS Linker
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.