Tested Applications
| Positive WB detected in | Transfected HEK-293T cells |
| Positive IHC detected in | Transfected HEK-293T cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Transfected HEK-293T cells, Transfected CHO-K1 |
| Positive FC detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:40000-1:160000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GGGGS Linker |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3745928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IF protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| IHC protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| WB protocol for GGGGS Linker antibody 98262-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-GGGGS Linker Rabbit Recombinant Antibody (242306C8), catalog number 98262-1-RR, has 1 total citation. The citation appears in the journal Microorganisms (Impact Factor 4.7), published in 2026. The associated research field is industrial microbiology and metabolic engineering, specifically involving sucrose isomerase mutant expression in Bacillus subtilis for isomaltulose production, where the antibody was applied in Western blot (WB).
| Species | Application | Title |
|---|---|---|
Microorganisms Sucrose Isomerase Mutants' Expression in Bacillus subtilis for Isomaltulose Production. |
Reviews
What customers say
The single review reports using this antibody as an ELISA coating reagent to detect a protein containing a (GGGGS)₄ motif at 1–10 µg/ml. The assay did not work, and technical support confirmed that (GGGGS)₄ detection is not supported, though (GGGGS)₃ detection is expected to perform well. Commercial 4GS antibodies from other vendors served as working positive controls. The reviewer rated the product 3 out of 5, reflecting the mismatch between the intended application and the antibody's validated capability.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniela (Verified Customer) (10-27-2025) | The antibody was used as coating antibody for an ELISA setup (tested 1 µg/ml, 10 µg/ml)d: 1 µg/ml, 5 µ to quantify a protein containing a (GGGGS)4 motif (0.5 µg/ml followed by detection using a b2m-biotin antibody) which was not working at all. According to the tech support, detection of a (GGGGS)4 motif is not working, but it would be working good for a (GGGGS)3 motif which could not be tested due to lack of material. A 4GS antibody from CST and from Genscript were used as positive controls that were both working.
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