GGTLC1 Polyclonal antibody

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  WB analysis of HepG2 using 17537-1-AP

  WB result of GGTLC1 antibody (17537-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGTLC1 transfected HepG2 cells.

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  WB analysis using 17537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17537-1-AP (GGTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17537-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GGTLC1 antibody (17537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

GGTLC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17537-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Gamma-glutamyltransferase light chain 1, Gamma-glutamyltransferase-like activity 4, Gamma-glutamyltransferase-like protein 6, GGTL6, GGTLA4

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, human urine
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17537-1-AP targets GGTLC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11592

  Product name: Recombinant human GGTLC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-225 aa of BC040904

  Sequence: MTSEFFSAQLRAQISDDTTHPISYYKPEFYMPDDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILLNNEMDDFSSTSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVGAAGGTQITMATALAIIYNLWFGYDVKWAVEEPRLHNQLLPNVTTVERNIDQEVTAALETRHHHTQITSTFIAVVQAIVRMAGGWAAASDSRKGGEPAGY

• Full Name: gamma-glutamyltransferase light chain 1
• Calculated Molecular Weight: 225 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC040904
• Gene Symbol: GGTLC1
• Gene ID (NCBI): 92086
• RRID: AB_3669277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BX51
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma-glutamyltransferase light chain 1 (GGTLC1), initially named GGTL6, then GGTLA4, and later GGTLC1 in 2008, is a member of the gamma-glutamyl transferase (GGT) family. The most ubiquitously expressed human GGT gene, GGT1, encodes a single transmembrane polypeptide that is post-translationally processed to form a heavy and a light chain. Knockdown of GGTLC1 in Endometrial cancer (EC) cells was shown to induce G0/G1 phase arrest and apoptosis, underscoring the significance of GGTLC1 as a key regulator of these cellular processes in EC (PMID: 38841516).