Growth Hormone Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

  Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82813-2-RR (Growth Hormone antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (Growth Hormone antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82813-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

Growth Hormone Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82813-2-PBS

Clone No.4G8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GH, GH1, 4G8, GH 1, GH-N

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Product Information

82813-2-PBS targets Growth Hormone in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0383

  Product name: recombinant human GH1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: N-6*HIS

  Domain: 27-217 aa of BC075012

  Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

• Full Name: GH1
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC075012
• Gene Symbol: GH1
• Gene ID (NCBI): 2688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01241
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.