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Growth Hormone Recombinant monoclonal antibody, PBS Only (82813-2-PBS)

Filter: AllWBIPIHCAffinity

WB analysis using 82813-2-RR (same clone as 82813-2-PBS)
Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
WB analysis using 82813-2-RR (same clone as 82813-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82813-2-RR (Growth Hormone antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
IP experiment of human placenta using 82813-2-RR (same clone as 82813-2-PBS)
IP result of anti-Growth Hormone (IP:82813-2-RR, 4ug; Detection:82813-2-RR 1:5000) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (Growth Hormone antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 82813-2-RR
Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

Growth Hormone Recombinant monoclonal antibody, PBS Only

Name: Growth Hormone Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 82813-2-PBS

Growth Hormone Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 82813-2-PBS

Clone No.4G8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GH, GH1, GH 1, GH-N, growth hormone 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

82813-2-PBS targets Growth Hormone in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0383 Product name: recombinant human GH1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: N-6HIS Domain: 27-217 aa of BC075012 Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF Predict reactive species
Full Name	GH1
Calculated Molecular Weight	217 aa, 25 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	BC075012
Gene Symbol	GH1
Gene ID (NCBI)	2688
RRID	AB_3086545
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01241
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.