GHR Polyclonal antibody

• Featured Product
• KD/KO Validated

GHR Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

5

Cat no : 20713-1-AP

Synonyms

GH binding protein, GH receptor, GHBP, GHR, Serum binding protein, Somatotropin receptor

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Validation Data Gallery

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  WB analysis of pig liver using 20713-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 20713-1-AP (GHR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig lung using 20713-1-AP

  pig lung tissue were subjected to SDS PAGE followed by western blot with 20713-1-AP (GHR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20713-1-AP (GHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20713-1-AP (GHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20713-1-AP (GHR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: pig liver tissue, pig lung tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below

Product Information

20713-1-AP targets GHR in WB, IHC, IF applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GH binding protein
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_000163
• Gene Symbol: GHR
• Gene ID (NCBI): 2690
• RRID: AB_2878726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature.

Publications

Species	Application	Title
human	WB	Pharmacol Res; Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.; Authors - Long Wang
human	WB	J Cell Mol Med; GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling.; Authors - Hong-Zhu Yan ; KD Validated
human	WB	Cell Signal; The inhibition of GHR enhanced cytotoxic effects of etoposide on neuroblastoma.; Authors - Hongmei Zhang
human	WB	FEBS Open Bio; PMID: 31725956; Authors - Mo Cheng ; KD Validated
human	WB	FEBS Open Bio; PMID: 32069380; Authors - Xiaojue Zhu ; KD Validated