GHRHR Polyclonal antibody

GHRHR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20715-1-AP

Synonyms

GHRFR, GHRH receptor, GHRHR, GHRHRpsv, GRF receptor, GRFR

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Validation Data Gallery

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  WB analysis of HEK-293 using 20715-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20715-1-AP (GHRHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20715-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20715-1-AP (GHRHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20715-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20715-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

20715-1-AP targets GHRHR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GHRH receptor
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000823
• Gene Symbol: GHRHR
• Gene ID (NCBI): 2692
• RRID: AB_11232423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GHRHR, also named GRFR, belongs to the G-protein coupled receptor 2 family. It is a GRF receptor coupled to G proteins that activate adenylyl cyclase. GHRHR stimulates somatotroph cell growth, GH gene transcription, and GH secretion. The antibody is specific to GHRHR. The antibody is specific to GHRHR.

Publications

Species	Application	Title
mouse	IF	Neural Regen Res; Growth hormone promotes the reconstruction of injured axons in the hypothalamo-neurohypophyseal system; Authors - Kai Li