GHRHR Polyclonal antibody

GHRHR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20715-1-AP

Synonyms

GHRFR, GHRH receptor, GHRHR, GHRHRpsv, GRF receptor, GRFR

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Validation Data Gallery

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  WB analysis of HEK-293 using 20715-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20715-1-AP (GHRHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20715-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20715-1-AP (GHRHR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20715-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 20715-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20715-1-AP (GHRHR Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20715-1-AP targets GHRHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GHRH receptor
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000823
• Gene Symbol: GHRHR
• Gene ID (NCBI): 2692
• RRID: AB_11232423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GHRHR, also named as GRFR, belongs to the G-protein coupled receptor 2 family. It is a receptor for GRF, coupled to G proteins which activate adenylyl cyclase. GHRHR stimulates somatotroph cell growth, GH gene transcription and GH secretion. The antibody is specific to GHRHR. The antibody is specific to GHRHR.