Tested Applications
| Positive WB detected in | human placenta tissue, SH-SY5Y cells, human peripheral blood platelets |
| Positive IHC detected in | human placenta tissue, human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue, human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24849-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20600 Product name: Recombinant human C10orf54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-196 aa of BC020568 Sequence: KVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAAL Predict reactive species |
| Full Name | chromosome 10 open reading frame 54 |
| Calculated Molecular Weight | 311 aa, 34 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC020568 |
| Gene Symbol | VISTA |
| Gene ID (NCBI) | 64115 |
| RRID | AB_2879755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7M9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VISTA antibody 24849-1-AP | Download protocol |
| IHC protocol for VISTA antibody 24849-1-AP | Download protocol |
| WB protocol for VISTA antibody 24849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VISTA (VSIR) antibody, catalog number 24849-1-AP, has a total of 5 citations. These appear in Frontiers in Immunology, Frontiers in Cell and Developmental Biology, Journal of Ovarian Research, Journal of Nutritional Biochemistry, and Immunooncology Technologies. The research fields span immune checkpoint inhibitor response prediction, tumor microenvironment profiling, ovarian cancer progression via NF-κB signaling, PD-L1 expression in gastric cancer, and T cell suppression through ligand-receptor interactions.
| Species | Application | Title |
|---|---|---|
Front Immunol Machine learning-driven prediction of immune checkpoint inhibitor responses against cholangiocarcinoma: a bile biopsy perspective. | ||
J Ovarian Res VISTA promotes ovarian cancer progression through activation of NF-κB pathway and tumor microenvironment reprogramming. | ||
Front Cell Dev Biol Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis. | ||
J Nutr Biochem Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression | ||
Immunooncol Technol Ligand-receptor interactions of V-domain Ig-containing suppressor of T cell activation and programmed death-1 suppress the anticancer activities of T cells. |
