Validation Data Gallery
|Positive WB detected in||HepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, A2780 cells|
|Western Blot (WB)||WB : 1:600-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14822-1-AP targets GIPC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GIPC1 fusion protein Ag6597|
|Full Name||GIPC PDZ domain containing family, member 1|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC000410|
|Gene ID (NCBI)||10755|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IHC protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IF protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IP protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Hum Genet
Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.
SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.
GIPC1 CGG repeat expansion is associated with movement disorders.
G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.
BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.
CNS Neurosci Ther
G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (11-02-2023)
Used at 1:2000 on PVDF membrane, overnight incubation at 4C in 3% BSA PBS 0.1% Tween. No non-specific bands and band reduced by knockdown with GIPC1 siRNA. Diluted antibody succesfully re0used after freezing at -20C
Sophy (Verified Customer) (01-27-2023)
I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.