GIPC1 Polyclonal antibody

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  WB analysis using 14822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14822-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14822-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14822-1-AP (GIPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14822-1-AP

  IP result of anti-GIPC1 (IP:14822-1-AP, 4ug; Detection:14822-1-AP 1:500) with mouse brain tissue lysate 9500ug.

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  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14822-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14822-1-AP (GIPC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14822-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIPC1 antibody (14822-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of A2780 using 14822-1-AP

  Immunofluorescent analysis of A2780 cells, using 14822-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

GIPC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14822-1-AP

Publications(12)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C19orf3, GAIP C-terminus-interacting protein, GIPC, PDZ domain-containing protein GIPC1, RGS19-interacting protein 1

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:600-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

14822-1-AP targets GIPC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIPC1 fusion protein Ag6597
• Full Name: GIPC PDZ domain containing family, member 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000410
• Gene Symbol: GIPC1
• Gene ID (NCBI): 10755
• RRID: AB_2263269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAIP interacting protein C terminus, member 1(GIPC1), also known as PDZ domain-containing protein GIPC1, is a cytoplasmic protein that also localizes to the peripheral membrane, acting as an adaptor protein linking receptor interactions to intracellular signaling pathways, including cell cycle regulation, and expression has been associated with some cancers (PMID: 19175293, 26757732). This antibody is specific to GIPC1 with a band at 42 kDa (PMID: 18721486).

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.; Authors - Jianwen Deng
human	IHC	Dev Cell; SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.; Authors - Christoph J Burckhardt
human	WB	Ann Neurol; GIPC1 CGG repeat expansion is associated with movement disorders.; Authors - Yu Fan
mouse	WB	Pain; G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.; Authors - Bao-Chun Jiang
mouse	WB	FASEB J; BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.; Authors - Nahoko Eiraku ; KD Validated
human,mouse	WB	CNS Neurosci Ther; G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.; Authors - Yong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-02-2023)

Used at 1:2000 on PVDF membrane, overnight incubation at 4C in 3% BSA PBS 0.1% Tween. No non-specific bands and band reduced by knockdown with GIPC1 siRNA. Diluted antibody succesfully re0used after freezing at -20C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HUVEC

GIPC1 Antibody Western Blot validation (1:2000 dilution) in HUVEC (Cat no:14822-1-AP)

FH

Sophy (Verified Customer) (01-27-2023)

I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.

• Applications: Western Blot
• Cell Tissue Type: NSCLC cells