• Featured Product
  • KD/KO Validated

GIPC1 Polyclonal antibody

GIPC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14822-1-AP

Synonyms

C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2



Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14822-1-AP targets GIPC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIPC1 fusion protein Ag6597
Full Name GIPC PDZ domain containing family, member 1
Calculated molecular weight 36 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC000410
Gene symbol GIPC1
Gene ID (NCBI) 10755
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GIPC1 antibody 14822-1-APDownload protocol
IHC protocol for GIPC1 antibody 14822-1-APDownload protocol
IF protocol for GIPC1 antibody 14822-1-APDownload protocol
IP protocol for GIPC1 antibody 14822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Am J Hum Genet

Expansion of GGC Repeat in GIPC1 Is Associated with Oculopharyngodistal Myopathy.

Authors - Jianwen Deng
humanIHC

Dev Cell

SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt.

Authors - Christoph J Burckhardt
humanWB

Ann Neurol

GIPC1 CGG repeat expansion is associated with movement disorders.

Authors - Yu Fan
mouseWB

Pain

G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.

Authors - Bao-Chun Jiang
mouseWB

FASEB J

BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.

Authors - Nahoko Eiraku
  • KD Validated
human,mouseWB

CNS Neurosci Ther

G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.

Authors - Yong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sophy (Verified Customer) (01-27-2023)

I ordered a GIPC1 antibody from another company before and the results were poor. Proteintech antibody worked very well with the dilution of 1:1000 in western.

  • Applications: Western Blot
  • Cell Tissue Type: NSCLC cells