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GIPC1 Antibody

GIPC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14822-1-AP

Synonyms

C19orf3, GIPC, GIPC1, GLUT1CBP, Hs.6454, IIP 1, NIP, RGS GAIP interacting protein, RGS19 interacting protein 1, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, Tax interaction protein 2, TIP 2



Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14822-1-AP targets GIPC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIPC1 fusion protein Ag6597
Full Name GIPC PDZ domain containing family, member 1
Calculated molecular weight 36 kDa
Observed molecular weight 42 kDa
GenBank accession number BC000410
Gene symbol GIPC1
Gene ID (NCBI) 10755
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GIPC1 antibody 14822-1-APDownload protocol
IHC protocol for GIPC1 antibody 14822-1-APDownload protocol
IF protocol for GIPC1 antibody 14822-1-APDownload protocol
IP protocol for GIPC1 antibody 14822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

GIPC proteins negatively modulate Plexind1 signaling during vascular development.

Authors - Jorge Carretero-Ortega
mouseWB,IF

Biol Reprod

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.

Authors - Przemysław Zakrzewski
mouseWB

FASEB J

BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.

Authors - Nahoko Eiraku
  • KD Validated