Validation Data Gallery
|Positive WB detected in||HepG2 cells, mouse skeletal muscle tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, A2780 cells|
|Western Blot (WB)||WB : 1:600-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14822-1-AP targets GIPC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GIPC1 fusion protein Ag6597|
|Full Name||GIPC PDZ domain containing family, member 1|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC000410|
|Gene ID (NCBI)||10755|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IHC protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IF protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|IP protocol for GIPC1 antibody 14822-1-AP||Download protocol|
|Click here to view our Standard Protocols|
GIPC proteins negatively modulate Plexind1 signaling during vascular development.
Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.
CNS Neurosci Ther
G-alpha interacting protein interacting protein, C terminus 1 regulates epileptogenesis by increasing the expression of metabotropic glutamate receptor 7.
BMP9 directly induces rapid GSK3-β phosphorylation in a Wnt-independent manner through class I PI3K-Akt axis in osteoblasts.
G protein-coupled receptor GPR151 is involved in trigeminal neuropathic pain through the induction of Gβγ/extracellular signal-regulated kinase-mediated neuroinflammation in the trigeminal ganglion.
GIPC1 CGG repeat expansion is associated with movement disorders.