Tested Applications
Positive WB detected in | HEK-293 cells, mouse testis tissue |
Positive IHC detected in | human cervical cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
11395-1-AP targets GKAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GKAP1 fusion protein Ag1914 Predict reactive species |
Full Name | G kinase anchoring protein 1 |
Calculated Molecular Weight | 366 aa, 42 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC014476 |
Gene Symbol | GKAP1 |
Gene ID (NCBI) | 80318 |
RRID | AB_2877761 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q5VSY0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GKAP1, or GKAP42, is a novel male germ cell-specific 42-kDa protein isolated by Yuasa K et al. GKAP42 mRNA is specifically expressed in testis, where it is restricted to the spermatocytes and early round spermatids.
Protocols
Product Specific Protocols | |
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WB protocol for GKAP1 antibody 11395-1-AP | Download protocol |
IHC protocol for GKAP1 antibody 11395-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |