GKAP1 Polyclonal antibody

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  WB analysis of HEK-293 using 11395-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11395-1-AP (GKAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 11395-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11395-1-AP (GKAP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11395-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11395-1-AP (GKAP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 11395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 11395-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 11395-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

GKAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11395-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

FKSG21, G kinase anchoring protein 1, GKAP1, GKAP42

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IHC detected in: human cervical cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11395-1-AP targets GKAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1914

  Product name: Recombinant human GKAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC014476

  Sequence: MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNSESDQCR

• Full Name: G kinase anchoring protein 1
• Calculated Molecular Weight: 366 aa, 42 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC014476
• Gene Symbol: GKAP1
• Gene ID (NCBI): 80318
• RRID: AB_2877761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VSY0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GKAP1, or GKAP42, is a novel male germ cell-specific 42-kDa protein isolated by Yuasa K et al. GKAP42 mRNA is specifically expressed in testis, where it is restricted to the spermatocytes and early round spermatids.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF,IHC	Mol Reprod Dev; Functional role of GKAP1 in the regulation of male germ cell spontaneous apoptosis and sperm number.; Authors - Hanshu Wang ; KO Validated