CoraLite® Plus 750 Anti-Mouse GL7 (GL-7)

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  FC experiment of mouse splenocytes using CL750-65261

  1X10^6 unstimulated or anti-CD3/CD28 stimulated mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 750 Anti-Mouse GL7 (CL750-65261, Clone:GL-7). Cells were not fixed.

GL7 Monoclonal Antibody for FC

Cat No. CL750-65261

Clone No.GL-7

Host / Isotype

Rat / IgM, kappa

Reactivity

mouse

Applications

FC

Synonyms

Ly77, lymphocyte antigen 77

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65261 targets GL7 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  LPS activated DBA/J mouse B cells

• Full Name: lymphocyte antigen 77
• Gene Symbol: GL7
• Gene ID (NCBI): 104230
• RRID: AB_3084877
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.