• Featured Product
  • KD/KO Validated

GLDC Polyclonal antibody

GLDC Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24827-1-AP

Synonyms

GCE, GCSP, GLDC, Glycine decarboxylase, HYGN1, NKH



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24827-1-AP is GLDC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLDC fusion protein Ag20410
Full Name glycine dehydrogenase (decarboxylating)
Calculated molecular weight 1020 aa, 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC111995
Gene symbol GLDC
Gene ID (NCBI) 2731
RRIDAB_2879746
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLDC(Glycine dehydrogenase [decarboxylating], mitochondrial) is also named as GCSP and belongs to the GcvP family. It induces dramatic changes in glycolysis and glycine/serine metabolism that leads to changes in pyrimidine metabolism to regulate cancer cell proliferation(PMID:22225612).

Protocols

Product Specific Protocols
WB protocol for GLDC antibody 24827-1-APDownload protocol
IHC protocol for GLDC antibody 24827-1-APDownload protocol
IP protocol for GLDC antibody 24827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Authors - Jiliang Xia
  • KD Validated
humanWB

J Biol Chem

De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

Authors - Teresa W M Fan
mouseWB

Life Sci Alliance

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Authors - Shengya Tian
  • KD Validated