Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24827-1-AP targets GLDC in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20410 Product name: Recombinant human GLDC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 675-1020 aa of BC111995 Sequence: NIDAVHLKAMVDKHKENLAAIMITYPSTNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPFLPNHPVISLKRNEDACPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKQATETAILNANYMAKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPENKFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS Predict reactive species |
| Full Name | glycine dehydrogenase (decarboxylating) |
| Calculated Molecular Weight | 1020 aa, 113 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC111995 |
| Gene Symbol | GLDC |
| Gene ID (NCBI) | 2731 |
| RRID | AB_2879746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23378 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GLDC(Glycine dehydrogenase [decarboxylating], mitochondrial) is also named as GCSP and belongs to the GcvP family. It induces dramatic changes in glycolysis and glycine/serine metabolism that leads to changes in pyrimidine metabolism to regulate cancer cell proliferation(PMID:22225612).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GLDC antibody 24827-1-AP | Download protocol |
| IP protocol for GLDC antibody 24827-1-AP | Download protocol |
| WB protocol for GLDC antibody 24827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GLDC Polyclonal antibody (Cat# 24827-1-AP) has 7 citations published in journals including Nature Communications, Science Translational Medicine, EMBO Molecular Medicine, Metabolites, Journal of Biological Chemistry, Life Science Alliance, and Pharm Sci Adv. Research fields span glycine metabolism in cancer (multiple myeloma, lung cancer, hepatocellular carcinoma), methylmalonic acidemia, IgA nephropathy, and pluripotent stem cell biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
| ||
Sci Transl Med Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin | ||
EMBO Mol Med Glycine decarboxylase advances IgA nephropathy by boosting mesangial cell proliferation through the pyrimidine pathway. | ||
Metabolites Differential Inhibition of Anaplerotic Pyruvate Carboxylation and Glutaminolysis-Fueled Anabolism Underlies Distinct Toxicity of Selenium Agents in Human Lung Cancer | ||
J Biol Chem De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues. | ||
Life Sci Alliance Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.
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Reviews
What customers say
The single review for 24827-1-AP is a 5-star rating from a user who tested the antibody at a 1/10 dilution (lot 00021248) on human corneal endothelial cells using a Simple Western application. The reviewer reported a strong and specific signal, indicating excellent performance for their use case.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
zhiguo (Verified Customer) (08-10-2026) | Simple Western application: Strong and specific signal
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