GLI2-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84141-1-RR (same clone as 84141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84141-1-RR (GLI2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84141-1-RR (same clone as 84141-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GLI2-Specific antibody (84141-1-RR, Clone: 241049C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

GLI2-Specific Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84141-1-PBS

Clone No.241049C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128

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Product Information

84141-1-PBS targets GLI2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GLI family zinc finger 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 80-90 kDa, 130-140 kDa, 160-180 kDa
• GenBank Accession Number: NM_005270
• Gene Symbol: GLI2
• Gene ID (NCBI): 2736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q0VGT2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.