GLI2-Specific Recombinant monoclonal antibody

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• GLI2-Specific Recombinant monoclonal antibody (84141-1-RR)

GLI2-Specific Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84141-1-RR Copied!

Clone No. 241049C10

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128

• 

  WB analysis using 84141-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84141-1-RR (GLI2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 84141-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84141-1-RR (GLI2-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 84141-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GLI2-Specific antibody (84141-1-RR, Clone: 241049C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, SKOV-3 cells, HT-1080 cells, A549 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84141-1-RR targets GLI2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GLI family zinc finger 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 80-90 kDa, 130-140 kDa, 160-180 kDa
• GenBank Accession Number: NM_005270
• Gene Symbol: GLI2
• Gene ID (NCBI): 2736
• RRID: AB_3671702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q0VGT2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

Publications

What published studies show

The GLI2-Specific Recombinant Monoclonal Antibody (241049C10), catalog number 84141-1-RR, has 1 total citation. The cited publication appeared in the journal Apoptosis (Impact Factor: 9). The research field is ferroptosis and bladder cancer, specifically investigating how GLI2 confers ferroptosis resistance by transcriptionally upregulating PRDX1.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Apoptosis; GLI2 confers ferroptosis resistance in bladder cancer by transcriptionally upregulating PRDX1.; Authors - Yuyang Yao