Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, Jurkat cells, mouse retina tissue, rat eye tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
32645-1-AP targets GLMN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37763 Product name: Recombinant human GLMN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 241-594 aa of BC001257 Sequence: ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHIDQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKSFLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFIIQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRYLVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSITVSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK Predict reactive species |
| Full Name | glomulin, FKBP associated protein |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC001257 |
| Gene Symbol | GLMN |
| Gene ID (NCBI) | 11146 |
| RRID | AB_3742689 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q92990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glomulin (GLMN) is a regulatory component of cullin-RING-based SCF (SKP1-Cullin-F-box protein) E3 ubiquitin-protein ligase complexes (PMID: 22405651; 22748924). It inhibits E3 ubiquitin ligase activity by binding to RBX1 (via the RING domain) and inhibiting its interaction with the E2 ubiquitin-conjugating enzyme CDC34 (PMID: 22405651; 22748924). GLMN is essential for normal vasculature development and contributes to the regulation of RPS6KB1 phosphorylation (PMID: 11845407; 11571281).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLMN antibody 32645-1-AP | Download protocol |
| WB protocol for GLMN antibody 32645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
