ZGLP1 Polyclonal antibody

ZGLP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55292-1-AP

Synonyms

GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1

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Validation Data Gallery

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  WB analysis of mouse ovary using 55292-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55292-1-AP (ZGLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 55292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 55292-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse ovary tissue
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

55292-1-AP targets ZGLP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GATA like protein-1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_001103167
• Gene Symbol: GLP-1
• Gene ID (NCBI): 100125288
• RRID: AB_2881301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.

Publications

Species	Application	Title
mouse	IHC	Front Pharmacol; Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell; Authors - Wenbin Wu