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  • KD/KO Validated

GLRX2 Polyclonal antibody

GLRX2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 13381-1-AP

Synonyms

GLRX2, Glutaredoxin 2, Glutaredoxin 2, mitochondrial, GRX2



Tested Applications

Positive WB detected inA549 cells, human heart tissue, mouse heart tissue
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13381-1-AP targets GLRX2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRX2 fusion protein Ag4218
Full Name glutaredoxin 2
Calculated molecular weight 164 aa, 18 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC028113
Gene symbol GLRX2
Gene ID (NCBI) 51022
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLRX2(Glutaredoxin-2) is also named as GRX2 and belongs to the glutaredoxin family. It facilitates the maintenance of cellular redox homeostasis upon treatment with apoptotic agents, thereby preventing cardiolipin oxidation and cytochrome C release. The GLRX2, which exists in a dynamic equilibrium of enzymatically active monomers and quiescent dimers,is a 16-kDa protein directed either to the nucleus or to the mitochondria through differential splicing of the first exon(PMID: 17121859). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GLRX2 antibody 13381-1-APDownload protocol
IF protocol for GLRX2 antibody 13381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Physiol Sci

Redox proteins are constitutively secreted by skeletal muscle.

Authors - Yasuko Manabe
mouseWB

Brain Res

Upregulation of Glutaredoxin 2 alleviates oxygen-glucose deprivation/reoxygenation-induced apoptosis and ROS production in neurons by enhancing Nrf2 signaling via modulation of GSK-3β.

Authors - Jian Wen
  • KD Validated
mouseWB

Int Immunopharmacol

Glutaredoxin 2 protects cardiomyocytes from hypoxia/reoxygenation-induced injury by suppressing apoptosis, oxidative stress, and inflammation via enhancing Nrf2 signaling.

Authors - Chengliang Li
humanWB

Free Radic Biol Med

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Authors - Kelei Dong
  • KD Validated
humanWB

Free Radic Biol Med

Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.

Authors - Meiling Wu