Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, mouse heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18884-1-AP targets Glutaredoxin 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13445 Product name: Recombinant human GLRX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC023528 Sequence: MSGSLGRAAAALLRWGRGAGGGGLWGPGVRAAGSGAGGGGSAEQLDALVKKDKVVVFLKGTPEQPQCGFSNAVVQILRLHGVRDYAAYNVLDDPELRQGIKDYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIHSTLLDEKKDQDSK Predict reactive species |
| Full Name | glutaredoxin 5 |
| Calculated Molecular Weight | 157 aa, 17 kDa |
| Observed Molecular Weight | 10-15 kDa |
| GenBank Accession Number | BC023528 |
| Gene Symbol | GLRX5 |
| Gene ID (NCBI) | 51218 |
| RRID | AB_3669306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86SX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Glutaredoxin 5 antibody 18884-1-AP | Download protocol |
| IHC protocol for Glutaredoxin 5 antibody 18884-1-AP | Download protocol |
| WB protocol for Glutaredoxin 5 antibody 18884-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Glutaredoxin 5 Polyclonal antibody (Cat# 18884-1-AP) has 3 citations. These appear in Cancer Letters, Experimental Cell Research, and Cell Molecular Biology Letters. The research fields span ferroptosis and metastatic lung adenocarcinoma, bladder cancer prognosis and cancer-associated fibroblast activation in the tumor microenvironment, and bone marrow stem cell senescence related to osteoporosis via CD36 S-glutathionylation. Applications cited include WB, IHC, and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Lett Hinokitiol preferentially suppresses metastatic lung adenocarcinoma via TMDD1-mediated ferroptosis induction and iron-sulfur cluster inhibition. | ||
Exp Cell Res GLRX5 is a prognostic marker in bladder cancer and correlates with activation of cancer-associated fibroblasts in the tumor microenvironment.
| ||
Cell Mol Biol Lett Grx2 deficiency accelerates BMSC senescence and senile osteoporosis via CD36 S-glutathionylation. |
