Glutaredoxin 5 Polyclonal antibody

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  WB analysis using 18884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18884-1-AP (Glutaredoxin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 18884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Glutaredoxin 5 antibody (18884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Glutaredoxin 5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18884-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

GRX5, Glutaredoxin-related protein 5, mitochondrial, GLRX5, GLRX 5, FLB4739

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, mouse heart tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18884-1-AP targets Glutaredoxin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13445

  Product name: Recombinant human GLRX5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-157 aa of BC023528

  Sequence: MSGSLGRAAAALLRWGRGAGGGGLWGPGVRAAGSGAGGGGSAEQLDALVKKDKVVVFLKGTPEQPQCGFSNAVVQILRLHGVRDYAAYNVLDDPELRQGIKDYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIHSTLLDEKKDQDSK

• Full Name: glutaredoxin 5
• Calculated Molecular Weight: 157 aa, 17 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC023528
• Gene Symbol: GLRX5
• Gene ID (NCBI): 51218
• RRID: AB_3669306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86SX6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation.