CoraLite® Plus 488-conjugated Glutaredoxin 5 Polyclonal antibody

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  IF Staining of MCF-7 using CL488-18884

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Glutaredoxin 5 antibody (CL488-18884) at dilution of 1:200.

Glutaredoxin 5 Polyclonal Antibody for IF/ICC

Cat No. CL488-18884

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

C14orf87, FLB4739, GLRX 5, GLRX5, Glutaredoxin-related protein 5, mitochondrial

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18884 targets Glutaredoxin 5 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13445

  Product name: Recombinant human GLRX5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-157 aa of BC023528

  Sequence: MSGSLGRAAAALLRWGRGAGGGGLWGPGVRAAGSGAGGGGSAEQLDALVKKDKVVVFLKGTPEQPQCGFSNAVVQILRLHGVRDYAAYNVLDDPELRQGIKDYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIHSTLLDEKKDQDSK

• Full Name: glutaredoxin 5
• Calculated Molecular Weight: 157 aa, 17 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC023528
• Gene Symbol: GLRX5
• Gene ID (NCBI): 51218
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86SX6
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation.