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GLUT1 Monoclonal antibody

GLUT1 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5A2

Cat no : 66290-1-Ig

Synonyms

DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1, SLC2A1,GLUT1



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lung cancer tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy GLUT1 Ready-To-Use IHC Kit. GLUT1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66290-1-Ig targets GLUT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GLUT1 fusion protein Ag17108
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Calculated molecular weight 492 aa, 54 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC121804
Gene symbol SLC2A1
Gene ID (NCBI) 6513
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate. 

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.


Protocols

Product Specific Protocols
WB protocol for GLUT1 antibody 66290-1-IgDownload protocol
IHC protocol for GLUT1 antibody 66290-1-IgDownload protocol
IF protocol for GLUT1 antibody 66290-1-IgDownload protocol
FC protocol for GLUT1 antibody 66290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomed Res Int

CD147 Expression Is Associated with Tumor Proliferation in Bladder Cancer via GSDMD.

Authors - Junming Peng
humanWB

Int J Mol Sci

EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma.

Authors - Irene Dell'Anno
mouseWB,IF

ACS Nano

Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Authors - Mengshi Jiang
mouseWB

J Food Sci

Chlorogenic Acid Stimulates the Thermogenesis of Brown Adipocytes by Promoting the Uptake of Glucose and the Function of Mitochondria.

Authors - Xue Han
humanWB

Immunobiology

The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus.

Authors - Fang Wang
humanWB

Reprod Biol Endocrinol

Downregulation of decidual SKP2 is associated with human recurrent miscarriage.

Authors - Shijian Lv
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (04-23-2019)

This antibody works ok-ish, even though the protocol says use 1:1500 to 1:3000, I have to use 1:500 to see the bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Fallopian tube epithelial cells