GLYCTK Polyclonal antibody

GLYCTK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12426-1-AP

Synonyms

HBEBP4, HBEBP2, HBeAgBP4A, HBeAg-binding protein 4, GLYCTK1

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Validation Data Gallery

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  WB analysis of human lung using 12426-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12426-1-AP (GLYCTK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12426-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12426-1-AP (GLYCTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12426-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12426-1-AP (GLYCTK antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLYCTK antibody (12426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human lung tissue, human liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12426-1-AP targets GLYCTK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLYCTK fusion protein Ag3097
• Full Name: glycerate kinase
• Calculated Molecular Weight: 523 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC021896
• Gene Symbol: GLYCTK
• Gene ID (NCBI): 132158
• RRID: AB_2110802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.