NeutraKine® GM-CSF Monoclonal antibody

NeutraKine® GM-CSF Monoclonal Antibody for Neutralization,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization,ELISA

Conjugate

Unconjugated

CloneNo.

1E10E7

Cat no : 69003-1-Ig

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim



Tested Applications

NeutralizationMeasured by its ability to neutralize Human GM-CSF induced proliferation of TF-1 cell line (human erythroleukemic cell line). The half neutralization dose (ND50) is typically 1.5-4 μg/mL in the presence of 0.2 ng/mL Human GM-CSF.

Product Information

69003-1-Ig targets NeutraKine® GM-CSF in Neutralization,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human NeutraKine® GM-CSF protein HZ-1002
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Gene symbol CSF2
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Lyophilized Powder
Purification Method Protein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody is used to Block/Neutralize GM-CSF protein.

Protocols

Product Specific Protocols
Neutralization protocol of NeutraKine® GM-CSF antibody 69003-1-IgDownload protocol
Standard Protocols
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