NeutraKine® GM-CSF Monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP50067-1

  Sandwich ELISA standard curve of MP50067-1, GM-CSF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69003-1-PBS. Detection antibody: 68702-1-PBS. Standard: Eg0189. Range: 7.8-500 pg/mL.

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  Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

  Recombinant human GM-CSF (Cat.NO. HZ-1002) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human GM-CSF (0.2 ng/mL HZ-1002) is neutralized by mouse anti-human GM-CSF monoclonal antibody 69003-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1.5-4 μg/mL This data was developed using the same antibody clone with 69003-1-PBS in a different storage buffer formulation.

NeutraKine® GM-CSF Monoclonal Antibody for Neutralization, Sandwich ELISA, Indirect ELISA

Cat No. 69003-1-PBS

Clone No.1E10E7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, Sandwich ELISA, Indirect ELISA

Synonyms

CSF2, GM-CSF, CSF 2, CSF, Colony-stimulating factor

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Product Information

69003-1-PBS targets NeutraKine® GM-CSF as part of a matched antibody pair:

MP50067-1: 69003-1-PBS capture and 68702-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  RecombinantProtein

• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• Gene Symbol: GM-CSF
• Gene ID (NCBI): 1437
• RRID: AB_2882921
• Conjugate: Unconjugated
• Purification Method: Protein A purification
• UNIPROT ID: P04141
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody can be used to neutralize the bioactivity of GM-CSF.