|Positive WB detected in
|rat kidney tissue, mouse kidney tissue
|Positive IP detected in
|Positive IHC detected in
|human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10864-2-AP targets GM2A in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GM2A fusion protein Ag1295
|GM2 ganglioside activator
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.
|Product Specific Protocols
|WB protocol for GM2A antibody 10864-2-AP
|IHC protocol for GM2A antibody 10864-2-AP
|IP protocol for GM2A antibody 10864-2-AP
|FC protocol for GM2A antibody 10864-2-AP
|Click here to view our Standard Protocols
Basic Res Cardiol
Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy.
Cell Struct Funct
Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.
Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity
Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis