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GM2A Polyclonal antibody

GM2A Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 10864-2-AP

Synonyms

Ganglioside GM2 activator, GM2 AP, GM2 ganglioside activator, GM2A, SAP 3



Tested Applications

Positive WB detected inrat kidney tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10864-2-AP targets GM2A in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GM2A fusion protein Ag1295
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_2110807
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

Protocols

Product Specific Protocols
WB protocol for GM2A antibody 10864-2-APDownload protocol
IHC protocol for GM2A antibody 10864-2-APDownload protocol
IP protocol for GM2A antibody 10864-2-APDownload protocol
FC protocol for GM2A antibody 10864-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Basic Res Cardiol

Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy.

Authors - Olga Eleftheriadou
humanWB

Cell Struct Funct

Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells.

Authors - Seiichiro Ishihara
  • KD Validated
humanWB

Mol Neurodegener

Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity

Authors - Yi-Chen Hsieh
humanWB

Nat Commun

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis

Authors - Sebastian Boland