GM2A Polyclonal antibody, PBS Only

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• GM2A Polyclonal antibody, PBS Only (10864-2-PBS)

• 

  WB analysis of rat kidney using 10864-2-AP (same clone as 10864-2-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 10864-2-AP (same clone as 10864-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

  IP result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GM2A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 10864-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

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Product Information

10864-2-PBS targets GM2A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1295

  Product name: Recombinant human GM2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of BC009273

  Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

• Full Name: GM2 ganglioside activator
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009273
• Gene Symbol: GM2A
• Gene ID (NCBI): 2760
• RRID: AB_2110807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17900
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.